FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206941
Sequences flagged as poor quality0
Sequence length197-201
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG6977233.71589003629054No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG5504426.598885672727974No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA155637.520501012365843No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA145037.008277721669462No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG98414.75546170164443No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG72553.5058301641530676No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA47922.315635857563267No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA41141.9880062433253922No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC28441.3743047535287836No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG24901.203241503616973No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG23651.142837813676362No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA14010.6770045568543691No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA13630.6586418351124234No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC10900.5267201762821287No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA7730.373536418592739No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG7680.3711202709951145No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA2530.12225706843979685No Hit
CCTGTTCGCTCCCCACGCTTTCGATCCTCAGCGTCAGTTACAGACCAGAG2360.11404216660787375No Hit
ACTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2150.10389434669785108No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC2070.10002851054165197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACCG151.3702338E-6447.23016195
GTAACCG151.3702338E-6447.23016195
CATTCCG102.4298916E-4447.23013195
TCTGCGG254.3655746E-11447.23013195
ATTGCCG2700.0447.23013195
AGTGCCG102.4298916E-4447.23013195
CACTCCG102.4298916E-4447.23013195
CTTGCCG254.3655746E-11447.23013195
CTGGCCG57000.0446.0532195
CCAGCCG10400.0442.92984195
TCTCCGG1650.0433.67767195
CCAACCG6150.0432.6861195
GAAGCCG1950.0412.82782195
TTCGCCG3050.0388.57697195
TAATCCG301.3096724E-10372.6918195
AAAGCCA301.3096724E-10372.6918195
CTGACCG158.1871694E-4298.15344195
CTGTCCG158.1871694E-4298.15344195
TCTACGG700.0255.56009195
CGTTCGC21400.0194.433793
Produced by FastQC (version 0.11.5)