FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85744
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG1961522.876236238104124No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTCATCCCAGGA1167613.617279343161037No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG1042512.158285127822355No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG60327.034894569882441No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA44385.175872364247061No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA37814.4096379921627165No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC36724.282515394663183No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA35924.1892144056727005No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG30503.557100205262176No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG24352.8398488523978354No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA12641.4741556260496362No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG11451.3353704049262922No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG10161.1849225601791378No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG9701.13127449150961No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA9471.1044504571748461No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG6530.7615693226348199No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA4260.4968277663743236No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACCTTCCCAAGG3920.4571748460533682No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG3460.40352677738384024No Hit
CCCATTTGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA2870.33471729800335887No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA2640.3078932636685949No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA2400.2799029669714499No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA2050.23908378428811344No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTTTCATCCCAGGA2020.23558499720097034No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA1690.19709833924239595No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA1560.18193692853144244No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTATTATCCCAGGG1480.1726068296323941No Hit
CCTGTTTGCTACCCACGCTTTCGCGCATGAACGTCAGTGTCATCCCAGGA1470.17144056727001306No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG1420.16560925545810787No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTAACGTTACGGTA1400.16327673073334578No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA1310.1527803694719164No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAATCCAGAG1310.1527803694719164No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTACATTCCCAAGG1270.14811532002239222No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTGTATCTTAGTG1260.1469490576600112No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACACGCCAGAG1180.13761895876096286No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGGCCAAAA1050.12245754805000934No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA1030.12012502332524726No Hit
CCTGTTCGCTCCCCCAGCTTTCGCACTTCAGCGTCAGTTGCCGTCCAGTG930.10846239970143683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGCCG153.7951067E-6346.29077195
ATTGCCG252.0008883E-10346.29077195
AAAGCCA153.7951067E-6346.29077195
GTAACCG13700.0346.29077195
TAATCCG202.752131E-8346.29074195
AAAACCG105.221639E-4346.29074195
CAAACGG105.221639E-4346.29074195
GTCACCG15950.0345.20523195
AAGTCCG2750.0339.99457195
CAGGCCG1250.0332.43912195
TAAACCA4650.0331.39655195
CCAACCG2000.0328.97623195
GGAACCG800.0324.64758195
CTGGCCG650.0319.653195
AAGGCCG700.0296.82065195
AAGTCCA150.0017585035230.86052195
GTTTGCT62100.0194.561264
GTTTGAT5250.0194.561264
CGATACC308.367351E-11194.561267
TCGATAC308.367351E-11194.561266
Produced by FastQC (version 0.11.5)