FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005301854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53416
Sequences flagged as poor quality0
Sequence length198-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG710213.295641755279316No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA48319.04410663471619No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA42337.924591882581998No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG40567.593230492736258No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA35916.7227048075483005No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG31075.816609255653737No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG24854.65216414557436No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA18013.3716489441365884No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGCTATCCCAGGG13532.532948929159802No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA13482.523588437921222No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG11762.201587539314063No Hit
CCTGTTCGCTCCCCGCACTTTCGCACCTCAGCGTCAATTATCTGCCGGTA10822.0256103040287554No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG10071.8852029354500526No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC9291.7391792721282013No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGACGGAGCCTGGTA8671.6231091807698068No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGA7891.4770855174479558No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTCCGAAACGGAA7031.3160850681443763No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA6861.2842593979332035No Hit
CCTGTTTGCTCCCCGCACCTTCGTATATCAGCGTCAATCATCGGCCAGAA4960.9285607308671558No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTAAGTTCCAGCA4910.9192002396285757No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC3930.7357346113524038No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG3290.6159203234985773No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG3190.5971993410214168No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTAACACTCCCGCA3080.5766062602965404No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC3010.5635015725625281No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGTGCTCCCGCA2860.5354200988467875No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTGACAGACCAGAG2820.5279317058559233No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATAAATTGTTAGTA2660.4979781338924667No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC2390.4474314812041336No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA1940.3631870600569118No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA1870.3500823723228995No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCCGTCCAGTG1810.3388497828366033No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTAACACTCCCGTA1800.3369776845888872No Hit
CCTGTTCGCTCCCCCAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG1630.30515201437771455No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA1620.3032799161299985No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG1590.29766362138685043No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA1550.2901752283959862No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGGCCAAAA1530.28643103190055413No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA1440.2695821476711098No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGCC1410.26396585292796165No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATCAATTGTTAGTA1400.2620937546802456No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA1320.2471169686985173No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGTGTCAGTTGCAGTCCAGAA1280.23962857570765314No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG1120.2096750037441965No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA1080.20218661075333233No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTGTATCTTAGTG1070.2003145125056163No Hit
CCTGTTTGATCCCCGCACTTTCGAGCCTCAGTGTCAGTTACAGTTTTGTG1070.2003145125056163No Hit
CCTGTTTGCTCCCCGCACCTTCGCACCTCAGCGTCAGTCGTCCGCCGGTA1070.2003145125056163No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTTCCGTCCAGTG1010.18908192301932006No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATTAATTGTTAGTA1000.18720982477160403No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA960.17972143178073985No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTATACCTTAGTA950.17784933353302382No Hit
CCTGTTTGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG940.17597723528530776No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAGTTGTATCTTAGTA900.1684888422944436No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG880.16474464579901155No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG860.16100044930357943No Hit
CCTGTTCGCTCCCCCAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG810.15163995806499925No Hit
CCTGTTCGATACCCGCACCTTCGAGCCTCAGCGTCAGTTACGCTCCGGAT710.13291897558783886No Hit
CCGGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAGTGGACCAGGT710.13291897558783886No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG690.12917477909240677No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTATTATCCCAGGG650.12168638610154262No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA610.11419799311067845No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGG600.1123258948629624No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC600.1123258948629624No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAATCCAGAG590.11045379661524636No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTGAGCGTCAGTAAAAGTCCAGAA590.11045379661524636No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGTCCAAAA580.10858169836753032No Hit
CCTGTTCGATACCCGCGCCTTCGAGCATAAGCGTCAGTTGCGCTCCCGCC560.10483750187209825No Hit
CCTGTTCGCTCCCCACGCTTTCGTCTCTCAGCGTCAATTGTCTGTTAGTC540.10109330537666616No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)