FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156826
Sequences flagged as poor quality0
Sequence length197-201
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2284814.56901279124635No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1822911.62371035415046No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA1713110.92357134658794No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA153419.782178975424994No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA133928.53940035453305No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA77074.91436368969431No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA69124.407432441049316No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA67184.28372846339255No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA63454.04588524861949No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA45112.876436305204494No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA43472.7718618086286715No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA37832.4122275643069386No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA23701.5112290053945137No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA21651.3805108846747352No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA19991.2746610893601826No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA18291.166260696568171No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA15701.0011095099026948No Hit
TACGGAAGGTCCTGGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA12590.80280055603025No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA12340.7868593217961307No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA11000.701414306301251No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA7720.49226531314960525No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA6460.4119214926096438No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA5520.35198245188935506No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA5010.3194623340517516No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA4090.26079859207019246No Hit
TACGTAGGGGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA3910.24932090342162655No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA3880.24740795531353219No Hit
TACATATGGGGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA3770.2403938122505197No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3220.20532309693545714No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA2990.19065716144006734No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA2610.16642648540420593No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA2260.14410875747643886No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA2090.1332687181972377No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1920.12242867891803656No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA1680.10712509405328198No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCAT650.0338.99195
AAGCGGG800.0338.99195
CAGGCAG203.0755473E-8338.99195
AAAGCAG105.573855E-4338.99195
AGATAGT105.573855E-4338.99195
AAGATAG20950.0337.37192195
AAGGCAG44500.0332.51492195
AGGCAGG650.0312.91385195
AAGGCGG14650.0274.2001195
AAGACAG150.0018770271225.99332195
ACATATG550.0194.573362
ATATGGG550.0194.573364
GTAGGGT1100.0194.573364
TAGGGTG1100.0194.573365
CATATGG550.0194.573363
GTCACGA256.379196E-9194.573358
GAGGATG950.0194.573355
AGGATGC950.0194.573356
GTCGCAA14350.0194.573358
GGTCCAG16150.0194.573358
Produced by FastQC (version 0.11.5)