FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101510
Sequences flagged as poor quality0
Sequence length198-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2747327.064328637572654No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1396313.755295044823171No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1388113.674514826125506No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA1326813.070633435129544No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA43084.243916855482219No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA40473.986799330115259No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA38033.746428923258792No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA32593.2105211309230617No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA18771.8490789084819228No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA13891.3683380947689883No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA12621.2432272682494334No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA10541.0383213476504778No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA9600.945719633533642No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA7980.78612944537484No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA7850.7733228253374052No Hit
TACGTAGGTCCCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA7650.7536203329721209No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGTGTCTA7490.7378583390798936No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA6380.6285095064525663No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA5960.5871342724854695No Hit
TACGGAGGATGCGAGCGTTATTCGGAATCATTGGGTTTAAAGGGTCTGTA3650.3595704856664368No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA3160.3112993793714905No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA2470.24332578071125996No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA1970.19406954979804947No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA1930.1901290513249926No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA1860.18323317899714314No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA1750.17239680819623682No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA1650.1625455620135947No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA1290.12708107575608313No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA1180.11624470495517683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATAG700.0438.57666195
AGGCAGG300.0438.57666195
CAGGCAG102.5721296E-4438.57666195
AAGGCAG24150.0426.77234195
AAGGCGG14250.0421.64914195
AAGCGGG1500.0409.3382195
AAGCCGG251.1351807E-5263.146195
GTCGCAA204.92053E-7194.442878
GATGCGA2400.0194.442878
ATGCGAG2400.0194.442879
CGTAGGG23550.0194.442873
TACGTAG83300.0194.442871
TCGCAAG204.92053E-7194.442879
TTAGGTC100.002944395194.442874
AGGTCCG153.8097176E-5194.442877
GGGGCTA100.002944395194.442878
TGGCGAG204.92053E-7194.442879
TGTCGCA204.92053E-7194.442877
GGGCTAG100.002944395194.442879
TCCCAAG1000.0194.442869
Produced by FastQC (version 0.11.5)