FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120117
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG3530229.389678396896358No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2820823.483769990925516No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG87687.29954960580101No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA66985.576229842570161No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG65445.448021512358784No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC61815.145816162574823No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC52884.402374351673784No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC35012.914658208246959No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG12571.046479682309748No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG10820.9007883979786375No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA10780.8974583114796406No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG9160.7625898082702698No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA8010.6668498214241114No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA7010.5835976589491912No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA5740.47786741260604243No Hit
CCCGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG5660.47120723960804883No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG4360.36297942839065245No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA3840.3196883039036939No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA3700.30803300115720506No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA3350.27889474429098293No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA3160.2630768334207481No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG3060.25475161717325606No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA2740.22811092518128157No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2540.21146049268629752No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTTCGTCCAGAA2240.18648484394382145No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG2010.16733684657458978No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA1770.1473563275806089No Hit
CCTGTTCTCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1630.13570102483412008No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA1620.13486850320937085No Hit
CCCGTTCGCTCCCCATGCTTTCGCACTCCAGCGTCGGTAGGGACCCAGAG1470.1223806788381328No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG1380.11488798421539No Hit
CCCGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG1310.10906033284214557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACCG500.0955.8914195
GTCACCG201.70985E-10955.8914195
GGCACCG50.008940882955.8914195
GAAACCG50.008940882955.8914195
GATACGG50.008940882955.8914195
GCTACCG50.008940882955.8914195
TATCCGG50.008940882955.8914195
TCGCCGG102.4818833E-5955.8914195
ATTGCCG50.008940882955.8914195
GATCCGT50.008940882955.8914195
AAAACCG102.4818833E-5955.8914195
TCTCCCG102.4818833E-5955.8914195
ATTCCCG50.008940882955.8914195
AAAGCCA800.0955.8914195
ACAACCG50.008940882955.8914195
GGAACCG1200.0955.89136195
ATATCCG1600.0896.1482195
TAATCCG750.0892.1653195
CTGGCCG650.0882.36127195
TCTCCGG5350.0875.4893195
Produced by FastQC (version 0.11.5)