FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93388
Sequences flagged as poor quality0
Sequence length199-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA4221145.199597378678206No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1647417.640382061935153No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1590717.033237675076027No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA1147012.282091917591126No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA17441.8674776202509957No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA4320.4625861995117146No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA3210.3437272454705101No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA2770.2966119844091318No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA1580.16918661926585857No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTTGTA1360.1456289887351694No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA1280.13706257763310062No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGAT50.00864105972.03217195
AAGATAG102.3558694E-5972.03217195
AAAGGCA50.00864105972.03217195
AGGCAGG156.079972E-8972.03217195
TGGCGGT50.00864105972.03217195
AAGCCGG50.00864105972.03217195
ATACAAA50.00864105972.03217195
GAAGGGA50.00864105972.03217195
GAAGGCA12300.0940.4214195
AAGCGGG1300.0934.64624195
AAGGCGG2150.0859.0052195
AAGGCAG404.984031E-9486.01608195
GAGGGCA552.4438123E-8353.46625195
GGCGCGA12250.0194.19828
AGGGCGC12250.0194.19826
GAGGTCC100.0029551315194.198185
GGATGCA100.0029551315194.198187
TACAGAG100.0029551315194.198181
ACGTCGG153.827914E-5194.198182
AGGATGC100.0029551315194.198186
Produced by FastQC (version 0.11.5)