FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93388
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG4068643.56662526234634No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1502916.093074056623976No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1479215.83929412772519No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG1164512.46948216044888No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG17241.846061592495824No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG11441.2249967875958367No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA10491.12327065575877No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG5060.5418255022058509No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG4090.4379577675932668No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA2840.30410759412344196No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1440.1541953998372382No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1330.1424165845718936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACCG1550.04262.1533195
AAACCGG54.4558023E-44262.153195
AATCCGG54.4558023E-44262.153195
TAAACCA1100.04262.153195
TAATCCG500.04262.153195
TGGATGA54.4558023E-44262.153195
TCTCCAG54.4558023E-44262.153195
TCTCCAA54.4558023E-44262.153195
TACTCGG54.4558023E-44262.153195
CCAACCG100.00178204862131.0764195
TTCGCCG100.00178204862131.0764195
AGGGCCG100.00178204862131.0764195
TAACCAA100.00178204862131.0764195
AAGGCCG100.00178204862131.0764195
AAAGCCA100.00178204862131.0764195
GAGACAA100.00178204862131.0764195
CAAACCA150.0040089971420.7177195
CGTTCGC15450.0194.045533
GTTTGCT19850.0194.045534
GTTTGAT204.968824E-7194.045534
Produced by FastQC (version 0.11.5)