FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69084
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1195617.30646748885415No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC889112.869839615540501No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG827511.978171501360663No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA40785.902958716924324No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG32834.752185744890278No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG26623.8532800648485903No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG23543.407446007758671No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA22463.2511145851427248No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC20923.028197556597765No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA17972.601181170748654No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG17942.596838631231544No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA17732.566440854611777No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA16582.3999768397892423No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA12091.7500434253951713No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA11451.6574025823634997No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA8611.2463088414104568No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG6590.9539111805917435No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGCTGTCCAGTA5870.8496902321811128No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA5470.7917897052863181No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG5020.726651612529674No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA4730.6846737305309478No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG4460.6455908748769614No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG4420.6398008221874819No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG4030.583347808465057No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC3660.5297898210873719No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA2880.4168837936425221No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA2880.4168837936425221No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTGTTGCCCAGAA2280.33003300330033003No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA2060.2981877135081929No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA2000.2895026344739737No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA1960.2837125817844942No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA1790.2591048578542065No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA1260.18238665971860343No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTCAGCGTCAGTTGCGCTCCCGTC1210.1751490938567541No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA1120.16212147530542528No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATCGTCCAGTT1030.14909385675409645No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA880.12738115916854845No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTTTGGTCCAGCA800.11580105378958948No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACACGCCAGAG730.1056684615830004No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACCG700.0399.7228195
GTAACCG900.0399.7228195
GGAACCG450.0399.7228195
CCAACCG300.0399.72278195
GTCACCG400.0399.72278195
GAAGCCG600.0399.72278195
CTGGCCG8000.0399.72278195
TCTGCGG103.3924665E-4399.72278195
TATCCAG103.3924665E-4399.72278195
ATTGCCG103.3924665E-4399.72278195
TAATCCG1250.0399.72278195
TCTCCGG3250.0393.5732195
ATATCCG1000.0379.73663195
CAAGCCG1750.0376.8815195
CACTCCG550.0363.38437195
CCAGCCG500.0359.75052195
TCCACGG2250.0346.42642195
ATTTCCG206.73081E-6299.79208195
TGTTCGC6850.0194.217253
TTTGCTA2600.0194.217255
Produced by FastQC (version 0.11.5)