FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133602
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC4303732.212841125132854No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2473818.516189877397046No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1658812.41598179667969No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA71435.346476849148965No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG67185.028367838804808No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG53634.0141614646487325No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA31622.3667310369605246No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA22571.6893459678747325No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG22291.66838819778147No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA15131.1324680768251973No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG10730.8031316896453646No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG8180.6122662834388707No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG7490.5606203499947605No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA7260.5434050388467239No Hit
CCCGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG5950.4453526144818192No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA4850.363018517686861No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTCAGCGTCAGTTGCGCTCCCGTC4280.32035448571129177No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA3350.2507447493300998No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2550.19086540620649392No Hit
CCTTTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC2500.18712294726126855No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2420.18113501294890796No Hit
CCTTTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2290.17140461969132198No Hit
CCCGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG2140.16017724285564588No Hit
CCTGTTCGCTACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC1830.13697399739524857No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGAA1460.10927980120058083No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGACCAGAG1460.10927980120058083No Hit
CCTGTTCGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA1450.10853130941153576No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTTCGTCCAGAA1450.10853130941153576No Hit
CCTGTTCGCTCCCCACGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG1360.10179488331013009No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGAG1340.10029789973203992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACCG700.0435.508195
ATTGCCG900.0435.50797195
TCTCCAG44750.0435.02133195
TAATCCG2200.0425.61008195
TTCGCCG4050.0424.75464195
CGAACCG1400.0404.40027195
CCAACCG550.0395.91635195
GGAACCG800.0381.06946195
TCTACAG350.0373.29257195
TCACCAG301.5097612E-10362.9233195
CCACCCG158.857549E-4290.33865195
AAAACCG306.643677E-8290.33865195
TCTCCCG158.857549E-4290.33865195
TCTCCAA158.857549E-4290.33865195
TCTCAAG302.4203235E-5217.75398195
CCTGTTC85850.0194.44651
CTCCCCA56600.0194.44659
CGTTCGA204.9258597E-7194.446493
CTTTTCG1200.0194.446492
GTTTGAT153.8121212E-5194.446494
Produced by FastQC (version 0.11.5)