FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301919

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301919
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120449
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3305527.4431502129532No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA2091017.360044500161894No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1567213.011315992660794No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA116159.643085455254921No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA62675.203031988642496No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA58854.885885312455894No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA39013.238715140847994No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA23941.9875632010228395No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA17961.4910875142176356No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA17311.4371227656518528No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA15641.298474873182841No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA15161.2586239819342626No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA12651.0502370297802388No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA10590.8792102881717574No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA8660.7189764962764323No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA5930.4923245523001436No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA5690.4723991066758545No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA4570.3794136937625053No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA4540.3769230130594691No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA4410.36613006334631254No Hit
TACGTAGGGGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA4390.3644696095442885No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA3950.32793962589975845No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA2960.24574716269956579No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA2470.20506604454997554No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA2230.18514059892568638No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA2140.17766855681657798No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA2050.17019651470746955No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1980.1643849264003852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGAG152.2113127E-6396.55548195
AAGATAG700.0396.55548195
AGGCAGG152.2113127E-6396.55548195
AGCCAGG103.4810035E-4396.55545195
AAGGCAG37750.0394.4545195
AAGCGGG3450.0385.0611195
AAGGCGG11650.0326.7753195
AAGGCGT150.0011726252264.37033195
AAGCCGG150.0011726252264.37033195
ACGTCGG100.0029429363194.490052
GTCGCGA100.0029429363194.490058
GGTCCTG100.0029429363194.490058
GTCGCAA800.0194.490058
GGTCCCA100.0029429363194.490057
TACGGAT100.0029429363194.490051
TCGCGAG100.0029429363194.490059
TACGTCG100.0029429363194.490051
AGGTCCT100.0029429363194.490057
ACGGATG100.0029429363194.490052
AAGTAGG153.807788E-5194.490042
Produced by FastQC (version 0.11.5)