FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163012
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1937911.888081859004245No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC1752110.748288469560523No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG129607.95033494466665No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG124747.65219738424165No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA114076.9976443452015795No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG100016.135131155988517No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG93285.722278114494638No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC69694.27514538806959No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG49343.026771035261208No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG46582.8574583466247883No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG32992.0237773906215493No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA29901.83422079356121No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA26491.6250337398473733No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG22241.3643167374181042No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA21091.293769783819596No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA20491.2569626775942875No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA19121.1729197850464996No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA15890.9747748632002552No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA15540.9533040512354919No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC13230.8115966922680539No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA11260.6907466934949574No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG10110.6201997398964493No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG9370.5748043088852355No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA9010.5527200451500504No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA7130.4373911123107501No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC6940.42573552867273573No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA5590.3429195396657915No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG4080.25028832233209825No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA3740.22943096213775674No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA3550.21777537849974235No Hit
CCTGTTTGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG3540.21716192672932055No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA2950.1809682722744338No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACACGCCAGAG2940.18035482050401197No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG2230.1367997448040635No Hit
CCGGTTCGCTCCCCACGCTTTCGTGCCTTAGCGTCAGAAATGGCCCAGTA2030.12453070939562731No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC2030.12453070939562731No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA1980.12146345054351827No Hit
CCCGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1840.11287512575761294No Hit
CCTGTTTGATACCCGCACCTTCGCGCTTAAGCGTCAGTTGCGCTCCCGTC1750.10735405982381664No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA1650.10121954211959856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACCG2950.0409.8158195
GAAACCG103.156194E-4409.81577195
TCTGCGG151.9414092E-6409.81577195
TCGCCGT103.156194E-4409.81577195
TAATCCG950.0409.81577195
GTAACCG13950.0409.81577195
ATTTCCG103.156194E-4409.81577195
CTGGCCG4850.0405.59085195
GGAACCG1550.0396.59595195
TCTCCGG4300.0390.75458195
ATATCCG1000.0389.32495195
CCAACCG2750.0387.46216195
TTCGCCG2850.0373.867195
TCGTCCG500.0368.83417195
TAAACCA2400.0367.12662195
CCAGCCG2850.0366.67728195
CAAGCCG2050.0359.83826195
TCTCCAG33400.0301.22687195
ACAGCCG150.0010632721273.2105195
GCAACCG309.0149115E-8273.2105195
Produced by FastQC (version 0.11.5)