FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268435
Sequences flagged as poor quality0
Sequence length198-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA6344323.634399389051353No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA3284812.236854359528378No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA263189.804235662264608No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA247499.219736621528488No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA211197.867453946020452No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA199707.439417363607577No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA104073.8769161994523813No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA95003.5390317954067094No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA86673.228714586398942No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA72762.710525825618865No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA44361.6525415836235962No Hit
TACGTAGGTCCCGAGTGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA31561.1757036154003764No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA18420.6861996386462272No Hit
TACGCAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA17860.6653379775364614No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA17180.6400059604746028No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA9200.3427272896604392No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA8890.3311788701175331No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA8860.33006128112950994No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA8800.32782610315346356No Hit
TACGCAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA8780.3270810438281148No Hit
TACGCAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA7960.2965336114888148No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA7370.2745543613910258No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA7320.27269171307765383No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA6940.258535585896027No Hit
TACGCAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA6920.2577905265706782No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA6560.24437945871440014No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA6490.2417717510756794No Hit
TACGCATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA6090.2268705645687038No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA5980.22277273827928548No Hit
TACGGAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3780.1408162124909196No Hit
TACGTAGGGCGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3440.1281502039599903No Hit
TACGCAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA3240.12069961070650251No Hit
TACGCAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA3120.11622925475440983No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA3070.11436660644103787No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA3050.1136215471156891No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA2860.10654348352487568No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA2840.1057984241995269No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCATAAAGGGCATCTA2840.1057984241995269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCGT153.9232746E-6343.78738195
AAGATAG22200.0343.78735195
ACGATAG105.3477025E-4343.78735195
AAGGCAG45900.0338.9189195
AAGGCGG46200.0317.3708195
AAGCGGG1950.0317.3422195
AGGCAGG550.0312.53397195
AAGACGG201.23707105E-5257.8405195
AAGCTAG500.0240.65115195
AAAGCAG150.0018009217229.19159195
GGCGCGA58150.0194.565228
TACGGAG3700.0194.565221
GCGCGAG58150.0194.565229
TACGTAG205700.0194.565221
CGTATGT21750.0194.565223
GTAGGTC82900.0194.565224
TAGGTCC81850.0194.565225
GTATGTG100.0029415414194.56524
GGATGTC100.0029415414194.56524
GGATGCA256.3937478E-9194.56527
Produced by FastQC (version 0.11.5)