FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268435
Sequences flagged as poor quality0
Sequence length197-201
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG6111922.76864045299607No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3058411.393447203233558No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG2874010.706502505261982No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG2810910.471436288114441No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG208677.773576471026505No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC184636.878015161957271No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG126064.696108927673366No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA108024.0240654162087655No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC52541.9572708476912473No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA52371.950937843425783No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA39711.4793152904800044No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA30611.1403132974463093No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG16960.6318103078957662No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA15350.5718330322051893No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG15340.5714605025425149No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG14420.537187773576471No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG12930.4816808538379868No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA11200.4172332221953173No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA8690.3237282768640453No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG8360.31143479799579044No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA8280.30845456069439525No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA7480.27865218768044403No Hit
CCTGTTCGCTCCCCACGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG5840.21755732300184402No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA5630.20973420008568183No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA5490.20451878480824037No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGACCAGAG4180.15571739899789522No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTCAGCGTCAGTTGCGCTCCCGTC4110.1531096913591745No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG3510.13075791159871106No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG3460.1288952632853391No Hit
CCCGTTCGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3260.12144467003185129No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC3030.11287648779034029No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTTGGCTAGCA2920.10877866150092201No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA2840.1057984241995269No Hit
CCCGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2800.10430830554882932No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGCCG21300.0595.73676195
TGAACCG101.02886785E-4595.73676195
GTCACCG700.0595.7367195
TCTGCGG154.3575346E-7595.7367195
CCAACCG7250.0579.3026195
GGAACCG1650.0577.68414195
GAAGCCG1600.0577.12195
TAATCCG1000.0565.9499195
TAAACCA4700.0557.711195
CCAGCCG5250.0556.02094195
TTCGCCG7200.0541.95496195
CATTCCG1150.0518.0319195
GTAACCG255.6152203E-9476.5894195
TATCCAG201.3753543E-6446.80255195
TCTACAG153.468069E-4397.1578195
CCACCCG253.3533051E-6357.44205195
AAAGCCA253.3533051E-6357.44205195
TCTCCCG452.6557245E-10330.96487195
TCTCCAG49550.0321.61368195
AAAACCG250.001601558238.2947195
Produced by FastQC (version 0.11.5)