FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135868
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG3395724.99263991521182No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG2090515.386257249683515No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG2036214.986604645685519No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG117408.640739541319515No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA87966.473930579680277No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG83076.114022433538435No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG64144.720758383136574No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA27812.046839579591957No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC14351.056172167103365No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC14231.0473400653575529No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA13751.0120116583743046No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG10530.7750169281950128No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG9640.7095121735802397No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACACGCCAGAG6620.48723761297730145No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA4290.315747637412783No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGACCAGAG3990.29366738304825274No Hit
CCTGTTCGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG3480.25613095062855123No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTATTATCCCAGGG3230.23773073865810934No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG3230.23773073865810934No Hit
CCTGTTTGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG3010.22153855212412046No Hit
CCTGTTCGCTCCCCACGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG2430.17885006035269524No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG2320.17075396708570084No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA1860.13689757706008773No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACCTTCCCAAGG1820.13395354314481703No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG1720.12659345835664026No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGAG1680.12364942444136957No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAATCCAGAG1640.12070539052609885No Hit
CCTGTTCGCTCCCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1540.11334530573792209No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGAG1460.10745723790738068No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1430.10524921247092767No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAACA50.00754491040.641195
AAACCGG50.00754491040.641195
CCAACCG1150.01040.641195
GGCACCG50.00754491040.641195
GATAACG50.00754491040.641195
TATCCAG50.00754491040.641195
TAATCCG154.6487912E-81040.641195
GTGACCG50.00754491040.641195
TCTCCGG50.00754491040.641195
CAGGCCG154.6487912E-81040.641195
TACTCGG50.00754491040.641195
GTAACCG1700.01040.641195
GGAACCG6400.01032.511195
GTCACCG7550.01026.8577195
TCTCCAG3700.01012.5157195
TAAACCA3000.01005.95294195
TAAACCG253.4197E-10832.5128195
GCAACCG156.489664E-5693.7607195
TAACCCA156.489664E-5693.7607195
CAGACCG456.4301275E-9462.50717195
Produced by FastQC (version 0.11.5)