FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148
Sequences flagged as poor quality0
Sequence length199-201
%GC53

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA18916.463414634146343No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG817.055749128919861No Hit
CCGGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAGTGGACCAGGT484.181184668989547No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGG433.7456445993031355No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG342.961672473867596No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG312.7003484320557494No Hit
CCTGTTTGATCCCCGCACTTTCGAGCCTCAGTGTCAGTTACAGTTTTGTG312.7003484320557494No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAATGAGCCAGTA292.5261324041811846No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT242.0905923344947737No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT242.0905923344947737No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG221.9163763066202089No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGAAAAGGTCCAGTC221.9163763066202089No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAGTATTGGTCCAGAC221.9163763066202089No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG201.7421602787456445No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG201.7421602787456445No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG191.6550522648083623No Hit
CCTGTTTGCTCCCCATGCTTTCGCACCTCAGTGTCAGTATTAGGCCAGAT191.6550522648083623No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA191.6550522648083623No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTGAGTGTCAGTATCTGTCCAGGG181.5679442508710801No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA161.3937282229965158No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA141.2195121951219512No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGTGTCAGTATGATGCCAGGA141.2195121951219512No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAATACCGGTCCAGGA141.2195121951219512No Hit
CCTGTTCGCTCCCCCAGCTTTCGTGCCTCAGCGTCAGGGTCTGTCCAGAT110.9581881533101044No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACTTGTCCAGCG100.8710801393728222No Hit
CCTGTTCGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG100.8710801393728222No Hit
CCCGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAATGTCGGTCCAGGA100.8710801393728222No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTACCAGCCTAGTC100.8710801393728222No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTGTTGACCCAGGG90.7839721254355401No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA80.6968641114982579No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA80.6968641114982579No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG70.6097560975609756No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCTGTCCAGTG60.5226480836236934No Hit
CCGGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCACAAATCGTCCAGGT60.5226480836236934No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCAGTCCAGTG60.5226480836236934No Hit
CCTGTTTGCTCCCCATGCTTTCGCACCTCAGTGTCAGTATTAGGCCATAT50.4355400696864111No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG50.4355400696864111No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG50.4355400696864111No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGTCCAGGG50.4355400696864111No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTACTGGTCCAGGT50.4355400696864111No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG40.34843205574912894No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC30.2613240418118467No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG30.2613240418118467No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG30.2613240418118467No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGGT30.2613240418118467No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA30.2613240418118467No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTCGTCCCAGGG30.2613240418118467No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTACAGGCCCAGGG20.17421602787456447No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCCGGCCCAGGG20.17421602787456447No Hit
CCGGTTTGCTCCCCACGCTTTCGCCCCTCAGCGTCAGAAGTGGACCAGGT20.17421602787456447No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC20.17421602787456447No Hit
CCTGTTCGCTCCCCACGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG20.17421602787456447No Hit
CCGGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGAAGCGGACCAGGT20.17421602787456447No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC20.17421602787456447No Hit
CCCATTCGCTCCCCTCGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA20.17421602787456447No Hit
CCTGTTCGATCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG20.17421602787456447No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA20.17421602787456447No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA20.17421602787456447No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG20.17421602787456447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAACCG350.0267.75195
CAAACCA109.2933583E-4267.75195
ACAACCG109.2933583E-4267.75195
CGATCCG157.122926E-6267.75195
ATCCCCA152.8532622E-5194.727289
GTTTGAT152.8532622E-5194.727284
TGATCCC152.8532622E-5194.727287
TGTTTGC600.0194.727283
TGTTTGA152.8532622E-5194.727283
GATCCCC152.8532622E-5194.727288
TTGATCC152.8532622E-5194.727286
TTTGATC152.8532622E-5194.727285
GTTTGCT650.0194.727264
CTCCCCA800.0194.727269
CGCTCCC253.0413503E-9194.727267
TGTTCGC203.0216506E-7194.727263
TGCTCCC650.0194.727267
CTGTTTG750.0194.727262
GCTCCCC900.0194.727268
CTGTTCG203.0216506E-7194.727262
Produced by FastQC (version 0.11.5)