FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46389
Sequences flagged as poor quality0
Sequence length195-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG857118.476362930867232No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA574012.373623057190281No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT559912.069671689409127No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG466710.060574705210287No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG31506.790402897238569No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA29466.350643471512643No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG27996.033758002974843No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC23225.005496992821574No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG19884.285498717368342No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA19264.151846342883011No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA9662.082390221819828No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG9652.0802345383603873No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT3890.8385608657224773No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGGA1340.2888615835650693No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1230.26514906551122036No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA1220.2629933820517795No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAA1070.23065813016016729No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGAA800.17245467675526524No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG560.12071827372868568No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG530.11425122335036322No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACCA2900.0260.51157195
AAAGCCG6200.0260.51157195
CAAACCA1800.0260.51157195
ACAACCG100.0012232163260.51157195
AAAACCG7200.0256.89334195
GAAACCG7150.0256.86804195
CGAACCG2200.0254.59084195
CGGGCCG1200.0238.80225195
AAGGCCG1050.0235.70094195
TGAACCA203.715918E-5195.38368195
ACATCCA1000.0195.38367195
GTTTGCT25750.0194.470354
CGTTCGC100.0029380126194.470343
CGTTTGC100.0029380126194.470343
ATCCCCA10100.0194.470349
GTTTGAT11150.0194.470344
CCTGTTT36700.0194.470341
CTCCCCA25350.0194.470349
TGATCCC10100.0194.470347
TGTTCGC9100.0194.470343
Produced by FastQC (version 0.11.5)