FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67616
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1386020.49810695693327No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA948514.027744912446757No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT855812.656767628963559No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG59988.870681495504023No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG49067.255679129200189No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC39155.790049692380501No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA35535.254673450070989No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG33094.893812115475627No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA22143.2743729294841457No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG20793.0747160435399907No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA12941.9137482252721252No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT11841.7510648367250354No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG4370.6462967345007099No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAA2350.34755087553241837No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2300.3401561760530052No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGGA1450.21444628490298154No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGAA1240.1833885470894463No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA1030.15233080927591103No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG920.1360624704212021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACCA5400.0282.32977195
CGAACCG1550.0282.32977195
GAAACCG11650.0282.32974195
CAACCCA109.6247287E-4282.32974195
CGGGCCG350.0282.32974195
TGAACCA900.0282.32974195
CAAACCA1850.0282.32974195
AAAACCG11050.0281.05225195
AAGGCCG1350.0271.8731195
AAAGCCG8100.0266.64478195
CCTGTTT54550.0194.414961
CTGTTTG54550.0194.414962
GTTTGCT38850.0194.414954
TTCGCTA4250.0194.414955
GATACCC1350.0194.414958
TGTTTGC39000.0194.414953
TGATACC1350.0194.414957
TTGATAC1350.0194.414956
ATACCCA1350.0194.414959
GCTCCCC38600.0194.414958
Produced by FastQC (version 0.11.5)