FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122681
Sequences flagged as poor quality0
Sequence length198-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA3342527.245457731841118No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA1758014.329847327622044No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1472912.00593408922327No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA109068.88972212486041No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA88747.2333939240795235No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA70955.783291626250193No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA70725.764543816890961No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA49894.066644386661341No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA33542.733919677863728No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA8400.6847026026850124No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA6170.5029303641150626No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA5190.4230483938018112No Hit
TACGCAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA4840.39451911868993567No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA4160.33909081275829184No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA3960.32278836983722015No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA2700.22008297943446825No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA2460.20052004792918218No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA2460.20052004792918218No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGCGAGCGCA2120.17280589496336027No Hit
TACGCAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA2100.1711756506712531No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA2100.1711756506712531No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA1870.1524278413120206No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA1800.1467219862896455No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA1730.1410161312672704No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA1680.13694052053700248No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA1590.1296044212225202No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGTTTAAAGGGAGCGTA1570.12797417693041302No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTTGTA1560.12715905478435943No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA1550.12634393263830584No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA1510.12308344405409151No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA1480.12063807761593076No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA1320.10759612327907338No Hit
TACGCAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1230.10026002396459109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATAG251.03682396E-10386.32877195
TAGGCAG103.7648706E-4386.32874195
AAGGCAG43650.0376.15057195
AAGCGGG1400.0358.73386195
AGGCAGG650.0326.89355195
AAGGCGG4650.0290.78506195
ACGGCAG207.743256E-6289.74655195
AAGCCGG207.743256E-6289.74655195
GTATGTC600.0194.503074
GTCACAA308.367351E-11194.503078
GTCGCGA351.8189894E-12194.503078
TATGTCA308.367351E-11194.503075
GGTCCAG10850.0194.503078
ACGTATG600.0194.503072
TCGCGAG351.8189894E-12194.503079
CGTAGGG17500.0194.503073
TACGCAG1200.0194.503071
TACGTAT600.0194.503071
CGTATGT600.0194.503073
AGGTCCA10850.0194.503077
Produced by FastQC (version 0.11.5)