FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91796
Sequences flagged as poor quality0
Sequence length198-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA2363125.742951762604033No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1580317.215347073946578No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA1573917.14562726044708No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA53065.78020828794283No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA52545.723560939474487No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA33873.689703255043793No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA31633.4456839077955466No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA28393.0927273519543332No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA22762.479410867575929No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA21352.3258094034598455No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA17811.9401716850407424No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA15191.654756198527169No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA12331.3431957819512832No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA4750.5174517408165933No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATCACTGGGCGTAAAGGGCACGCA4360.4749662294653362No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA4000.43574883437186807No Hit
TACGTATGTCACGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA2670.29086234694322194No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA2370.25818118436533183No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2330.25382369602161314No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA2210.2407512309904571No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA1880.20480195215477798No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA1780.1939082312954813No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA1750.19064011503769226No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA1710.18628262669397358No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGGGCACGCA1110.12092030153819339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATAG1300.0268.86157195
AGGCAGG309.094947E-12268.86154195
AAGGCAG48650.0266.37466195
AAGGCGG10500.0254.77832195
GGGGCAA650.0194.723188
ATGGGGC100.0029312738194.723166
AGGGGGC1050.0194.723166
GTATGGG100.0029312738194.723164
GGATGCG3550.0194.723167
GTCACGA153.786714E-5194.723168
CGGAGGA3650.0194.723163
GTCACAA5500.0194.723168
GAGGATG3550.0194.723165
GATACGA100.0029312738194.723168
AGGATGC3550.0194.723166
GTCGCGA1450.0194.723168
GGCGCGA5500.0194.723168
GGTCCCG9000.0194.723167
TACGGGG100.0029312738194.723161
TATGTCG1350.0194.723165
Produced by FastQC (version 0.11.5)