FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94420
Sequences flagged as poor quality0
Sequence length198-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2699428.589281931794115No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1268713.43677187036645No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA56315.9637788604109305No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA54045.7233636941325985No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA52225.530607922050413No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA47315.010590976488032No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA37313.9514933276848123No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA35973.8095742427451813No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA29423.1158652827790725No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA27262.8871001906375766No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA17321.8343571277271764No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA14391.5240415166278332No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA11571.2253759796653252No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA9471.002965473416649No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA7130.7551366235966956No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA6940.7350137682694344No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA6720.7117136199957635No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA6300.6672315187460284No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA5360.5676763397585258No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA5230.5539080703240838No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA4480.47447574666384235No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA3860.40881169243804283No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA3780.400338911247617No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA3710.3929252277059945No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA3560.37703876297394623No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA3460.36644778648591403No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA3220.3410294429146367No Hit
TACGGGGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA3170.3357339546706206No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGAGTGCGCA2930.31031561109934336No Hit
TACGTATGTCACGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA2310.24465155687354376No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA1980.20970133446303751No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA1700.18004660029654734No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1580.1673374285109087No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA1510.15992374496928616No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA1450.15356915907646684No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1290.13662359669561533No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA1270.1345054013980089No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA1100.11650074136835417No Hit
TACGTAGGGTGCAAGCGTTGTCCGGATTTACTGGGCGTAAAGAGCTCGTA1050.11120525312433806No Hit
TACGTAGGGCGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1040.11014615547553484No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGTTTAAAGGGAGCGTA990.10485066723151876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATAG2750.0404.37775195
AAGGCAG23500.0392.33246195
AAGGCGG10700.0330.68274195
AGGCAGG450.0314.51602195
AAGGCAA150.0011051634269.58517195
AAGGCAT352.069064E-7231.073195
AAGCGGG1800.0224.65431195
GTCGCGA1350.0194.47838
TCGCGAG1350.0194.47839
AGGTCCG5700.0194.47837
ATGGGGC100.0029424655194.478296
AGGGGGC2500.0194.478296
GTCACGA400.0194.478298
GTCACAA750.0194.478298
GGCGCGA11200.0194.478298
GGCGCAA4200.0194.478298
CACGTAA204.914273E-7194.478291
TACGGGG500.0194.478291
ACGTAAG204.914273E-7194.478292
TGTCACG400.0194.478297
Produced by FastQC (version 0.11.5)