FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184237
Sequences flagged as poor quality0
Sequence length198-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA7394640.13634611940056No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA2810015.252093770523837No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA2057411.167137979884604No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA153798.34740035931979No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA90274.8996672763885645No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA69093.7500610626529958No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA32941.7879144797190574No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA30041.6305085297741495No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA29691.6115112599532122No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA11380.6176826587493283No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA9720.5275813218843121No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTTGTA9120.49501457361984835No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA7770.42173939002480504No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA5950.3229535869559318No Hit
TACGTAGGGCGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA5570.3023279797217714No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA5320.2887585012782449No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA5280.2865873847272806No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA4690.25456341560055795No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA4630.2513067407741116No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA4270.23176669181543338No Hit
TACGTAGGTGGCAAGCGTTGTCCAGATTTATTGGGCGTAAAGCGAGCGCA3960.21494053854546044No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA3730.20245661837741605No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA3400.184544906831961No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA3400.184544906831961No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGCTTGTA3380.18345934855647889No Hit
TACGTAGGTCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA3320.18020267373003251No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA2980.1617481830468364No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGAGCTTGTA2680.14546480891460456No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA2660.14437925063912244No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA2630.14275091322589925No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA2410.1308097721955959No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA2360.12809587650689058No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA2360.12809587650689058No Hit
TACGTAGGGTGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2010.10909860668595342No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA2000.10855582754821236No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA1930.10475637358402493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATAG252.1827873E-11504.65894195
AAGGCAT204.2127795E-9504.6589195
AAGTCGA101.6910565E-4504.6589195
AAGCGGG1000.0479.426195
AAGGCGG11650.0444.0132195
AGGCAGG1800.0434.5674195
AAGGCAG12800.0421.8633195
AGGCGGG750.0370.0832195
GAAGGCA42850.0350.96466195
GACGGCA303.1890522E-8336.4393195
GTATGTC308.367351E-11194.384114
GAGGATG308.367351E-11194.384115
AGGATGC308.367351E-11194.384116
GTCGCAA308.367351E-11194.384118
GTTCCCG153.8193397E-5194.384117
TATGTCG153.8193397E-5194.384115
TATGTCC153.8193397E-5194.384115
TTCCCGA153.8193397E-5194.384118
GACGTAG153.8193397E-5194.384111
CGTATGT308.367351E-11194.384113
Produced by FastQC (version 0.11.5)