FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113422
Sequences flagged as poor quality0
Sequence length196-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA4323238.116062139620176No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1780715.69977605755497No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1433512.638641533388583No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1388512.241893107157342No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA102809.063497381460387No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA22131.951120593888311No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA17041.5023540406623053No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA8890.7837985575990549No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA6710.5915959866692528No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA4980.4390682583625752No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA4750.4187900054663117No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA3920.3456119624058825No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA3870.34120364655886865No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA2140.18867591825219093No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTTGTA2020.17809596021935778No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1730.1525277283066777No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA1510.13313113857981695No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA1480.13048614907160866No Hit
TACGTAGGTCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA1420.12519617005519212No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA1390.12255118054698383No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA1370.12078785420817831No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA1370.12078785420817831No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA1350.11902452786937277No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA1320.1163795383611645No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGTTTAAAGGGAGCGTA1270.11197122251415069No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1250.11020789617534517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCAGG257.2759576E-12605.37775195
AAGGCAG17200.0589.53937195
AAGCGGG800.0529.7055195
AAGGCGG3650.0456.1065195
GAAGGCA28350.0238.09389195
GGTCCAG14750.0194.31888
ACGGAAG14750.0194.31882
GGCGCGA12400.0194.318798
GGTCCCG59950.0194.318797
ACGTAGG95800.0194.318792
GCGCGAG12500.0194.318799
AGGTGGC20600.0194.318796
TACGTAG95950.0194.318791
AAGGTCC14650.0194.318796
AGGTCCC59950.0194.318796
GTAGGTG20700.0194.318794
GTAGGGC12550.0194.318794
GGTGGCA20600.0194.318797
GGGGCAA1350.0194.318798
TAGGGCG12450.0194.318795
Produced by FastQC (version 0.11.5)