FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113422
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG3757133.12496693763114No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1767815.586041508702014No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC1384112.20309992770362No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1367112.053217188905151No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG108079.528133871735642No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG36783.242757137063356No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG19131.6866216430674825No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG16311.4379926292959038No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG12721.1214755514803125No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA7780.6859339457953483No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA5120.4514115427342138No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG4120.3632452257939377No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA4060.3579552467775211No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3110.2741972456842588No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA2670.23540406623053728No Hit
CCTGTTTGCTACCCACGCTTTCGAGCTTCAGCGTCAGTTACAGACCAGAG2240.19749254994621854No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA1860.1639893495089136No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG1500.1322494754104142No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1500.1322494754104142No Hit
CCTGTTCGCTCCCCACGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG1400.12343284371638659No Hit
CCTGTTTGATACCCGCACCTTCGCGCTTAAGCGTCAGTTGCGCTCCCGTC1390.12255118054698383No Hit
CCCGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1370.12078785420817831No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC1290.11373454885295622No Hit
CCTGTTCGATCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1250.11020789617534517No Hit
CCTGTTCGCTCCCCACGCTTTCGATCCTCAGCGTCAGTTACAGACCAGAG1220.10756290666713687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACCG1800.01059.7206195
TAAACCA500.01059.7205195
TAGCCCG50.0072725661059.7205195
CATTCCG50.0072725661059.7205195
TCGCCAG50.0072725661059.7205195
TAATCCG201.0186341E-101059.7205195
TCACCAG50.0072725661059.7205195
TCTCCGG50.0072725661059.7205195
TCTCCAG14650.01056.1036195
GAAGCCG700.0984.0261195
GTCACCG1500.0918.42444195
CCAACCG201.3617137E-7794.79034195
CCTGTTT39800.0193.90831
CCTGTTC55000.0193.90831
CTCCCCA76000.0193.90839
TGTTCGC54700.0193.90833
TGTTTGC24800.0193.90833
TGTTTGA15000.0193.90833
CCGTTCG18000.0193.90832
TGATACC14850.0193.90837
Produced by FastQC (version 0.11.5)