FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85284
Sequences flagged as poor quality0
Sequence length197-201
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1187313.92172036958867No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT1115013.073964635805075No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG73768.648750058627645No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA68167.992120444632053No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG67447.907696637118335No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG60417.0833919609774405No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG52436.147694761033723No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG50375.906148867313916No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA43545.105295248815722No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC37784.42990478870597No Hit
CCTGTTTGCTACCCATGCTTTCGCTCCTCAGCGTCAGTAACTGCCCAGTA32063.7592045401247596No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA16691.9569907602832888No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT14351.6826133858637027No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2490.2919656676516111No Hit
CCTGTTCGCTCCCCCCGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG2300.2696871628910464No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGGA2200.2579616340696965No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAG2130.24975376389475165No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA1770.20754186013789222No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAA1580.18526335537732752No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGAA1460.1711927207917077No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAA1450.17002016790957272No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1410.1653299563810328No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1410.1653299563810328No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATGATGCCAGGG1300.15243187467754796No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1220.1430514516204681No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1140.1336710285633882No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT1020.11960039397776841No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA1010.11842784109563341No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAC980.11491018244922846No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG950.11139252380282351No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTTGCA910.10670231227428358No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGTA860.10083954786360866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCCA209.0691174E-8272.7872195
GCAACCG100.0010676618272.7872195
AAAACCG8500.0272.7872195
ACAACCG100.0010676618272.7872195
ATAACCA6450.0272.78717195
CGGGCCG4400.0272.78717195
CGAACCG5950.0270.49484195
GAAACCG10450.0268.87155195
AAAGCCG11950.0268.2217195
CAAACCA2600.0267.5413195
TGAACCA1450.0263.38074195
AAGGCCG1000.0245.50845195
CCAACCA203.1040803E-5204.5904195
GTTTGCT45450.0194.710864
GATACCC1650.0194.710868
TGATCCC13150.0194.710867
TGTTTGA14800.0194.710863
TGATACC1650.0194.710867
TGCTCCC33500.0194.710867
TTGATAC1650.0194.710866
Produced by FastQC (version 0.11.5)