FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005301997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005301997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67026
Sequences flagged as poor quality0
Sequence length198-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1223118.24814251186107No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA906613.526094351445709No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA740611.049443499537492No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA55038.210246769910185No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA53427.970041476441978No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA51867.737295974696387No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA42416.327395339122131No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA38705.773878793304091No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA26233.913406737683884No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA22913.418076567302241No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA16242.4229403515053862No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA14492.1618476412138574No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA9571.4278041357085312No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGTA2010.2998836272491272No Hit
TACGCAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1500.22379375167845317No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGAGCGCA1300.19395458478799274No Hit
TACGCAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA1210.18052695968728555No Hit
TACGCAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA1200.1790350013427625No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA1070.15963954286396326No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGCGCGTA1040.15516366783039418No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGTA980.14621191776325607No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA910.13576820935159492No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA900.13427625100707188No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGTA870.12980037597350283No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA850.12681645928445678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCAG30500.0213.03336195
AAGGCGG21650.0210.42636195
AAGGCGA8400.0205.76234195
GGTCCCG5700.0194.911217
AGGTGGC23900.0194.911216
GTCCCGA5700.0194.911218
AGGTCCC5700.0194.911216
GTAGGTC5500.0194.911214
GTGGCAA23900.0194.911218
TCCCGAG5700.0194.911219
TAGGTCC5450.0194.911215
CGGAGGT153.7676447E-5194.91123
CGGAGGA6350.0194.91123
GTGCAAG19000.0194.91129
GAGGATC6350.0194.91125
ACAGAGG14950.0194.91122
TACAGAG14950.0194.91121
AGGATCC6350.0194.91126
GGCGCAA153.7676447E-5194.91128
GCAGGTG450.0194.91124
Produced by FastQC (version 0.11.5)