FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005302007

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302007
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197812
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA5534427.978080197359105No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA4216821.317210280468323No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA2997915.155298970739894No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA175718.882676480698844No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA87834.440074414090146No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA76743.879441085475097No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA63533.211635290073403No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA38461.9442703172709443No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA26601.3447111398701799No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA21931.1086283946373323No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA18550.9377590843831517No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA17010.8599073868117202No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA8370.4231290316057671No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA7550.3816755303014984No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA7310.3695427982124441No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA6900.34881604756030976No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA6750.3412330900046509No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA4730.2391159282551109No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA4490.22698319616605667No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA2980.15064809010575697No Hit
TACGTAGGTCCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA2870.14508725456494045No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA2810.14205407154267688No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA2660.134471113987018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGAT204.109097E-9507.23746195
CAGGCAG204.109097E-9507.23746195
AAGGCAG49800.0500.1076195
AAGCGGG6200.0490.87497195
AAGGCGG9400.0434.38953195
AGGCAGG202.6130692E-6380.4281195
ACGGCAG155.6132744E-4338.15833195
AAGCTGG155.6132744E-4338.15833195
AGGCGAG256.369539E-6304.3425195
GAGGCGG352.4392395E-5217.38748195
AAGCGGT250.0025910824202.89499195
GTCCCGA62000.0194.381218
GGTTGCA100.0029492974194.38127
GTGTCAA100.0029492974194.38128
TCCCAAG100.0029492974194.38129
GAGGATG100.0029492974194.38125
ACGTCGG256.4228516E-9194.38122
AGGATGC100.0029492974194.38126
GGCGCGA7050.0194.38128
TTCCGAG100.0029492974194.38129
Produced by FastQC (version 0.11.5)