FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005302021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116165
Sequences flagged as poor quality0
Sequence length198-201
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA2296119.76585029914346No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA1999817.21516807988637No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA1162010.00301295570955No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA108329.32466749881634No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA100498.650626264365343No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA57524.951577497525072No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA54334.676968105711703No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA36093.1067877587913744No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA34342.956139973313821No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA27182.339775319588516No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA14191.221538329100848No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGAGCATCTA13641.1741918822364739No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA11400.9813627168252055No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA9670.8324366203245384No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA8280.7127792364309388No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA8000.6886755907545301No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA6410.5518013170920673No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA6240.5371669607885335No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA4270.3675805965652305No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2850.24534067920630137No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA1930.16614298626953042No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1870.16097791933887143No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGAGCGCA1800.1549520079197693No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCATAAAGGGCATCTA1720.14806525201222398No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA1560.13429174019713339No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA1560.13429174019713339No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA1520.13084836224336072No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGCA1500.12912667326647442No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA1360.11707485042827014No Hit
TACGTAGGGGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA1230.10588387207850901No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGCA1210.10416218310162269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCGAG154.843916E-6325.91708195
AAGATGG106.267628E-4325.91708195
AAGACAG106.267628E-4325.91708195
ACGGCAG106.267628E-4325.91708195
AGATAGT154.843916E-6325.91708195
AAGATAG27650.0324.14902195
AAGGCAG20400.0316.3313195
AAGCGGG2850.0314.48138195
AGGCAGG251.1373231E-7260.73367195
AAGGCGG12250.0259.4034195
AAGGTAG253.719346E-5195.55025195
TAGTGCG100.0029380016194.596225
ACGTCGG100.0029380016194.596222
GGTCCTG100.0029380016194.596228
GGTCCCG23700.0194.596227
TATGTCA650.0194.596225
GGTCCAG5650.0194.596228
AGGGCCC100.0029380016194.596226
ACGTAGT100.0029380016194.596222
CCGTATG100.0029380016194.596222
Produced by FastQC (version 0.11.5)