FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005302022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116165
Sequences flagged as poor quality0
Sequence length197-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2214519.06340119657384No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG1409212.13102053114105No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG107829.281625274394182No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG97398.383764472947961No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG65695.654887444583136No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC64665.56622046227349No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC42563.6637541428141005No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG36313.1257263375371243No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA34582.976800241036457No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG33052.845091034304653No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG26962.3208367408427666No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC20421.7578444454009383No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA10530.9064692463306503No Hit
CCCGTTCGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTTTCGTCCAGAA9230.7945594628330391No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA9150.7876727069254938No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA8990.7738991951104033No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA8930.7687341281797443No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA7670.6602677226359058No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG7320.6301381655403951No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA7310.629277321051952No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG7030.6051736753755435No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG5930.5104807816467956No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG5590.48121206903972796No Hit
CCTGTTTGCTCCCCTAGCTTTCGTACTTCAGCGTCAGTTACCGTCCAGTG5420.46657771273619425No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA5380.4631343347824215No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA5140.44247406705978565No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA4640.3994318426376275No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA4230.36413721861145787No Hit
CCTGTTTGCTCCCCTAGCTTTCGTACTTCAGCGTCAGTTGCTGTCCAGTG4150.35725046270391253No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG3650.3142082382817544No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2870.2470623681831877No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG2840.2444798347178582No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTCAGCGTCAGTTGCGCTCCCGTC2410.20746352171480223No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA2300.19799423234192742No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2150.18508156501527998No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTAACACTCCCGTA2140.18422072052683683No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTCAGCGTCAGTTGCGCTCCCGTC1980.1704472087117462No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTTGCGCTCCCGTC1820.15667369689665564No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1760.15150862996599665No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA1680.14462187405845134No Hit
CCTGTTCGCTCCCCATGCTTTCGTTTCTCAGCGTCAGTTACAGCCCAGAG1500.12912667326647442No Hit
CCTGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1500.12912667326647442No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC1450.12482245082425861No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAAGCCAGAG1440.12396160633581542No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGAA1230.10588387207850901No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA1210.10416218310162269No Hit
CCTGTTTGCTCCCCACGCCTTCGTGCCTCAACGTCAGTTATCGTCCAGTA1210.10416218310162269No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)