FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005302031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147189
Sequences flagged as poor quality0
Sequence length198-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA2461316.72203765227021No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA2287615.541922290388548No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1852112.583141403229861No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA145199.864188220587137No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA92086.2559022753059No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA81295.522831189830762No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA70874.814897852421037No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA67624.594093308603225No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA57513.907221327680737No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA40822.7733050703517246No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA25511.7331458193207372No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA24751.6815115259971873No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA24341.6536561835463248No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA13830.9396082587693373No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA12970.8811799794821624No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA12280.83430147633315No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTCATTGGGCGTAAAGCGCTCGTA6620.449761870791975No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA5560.37774561957754993No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA3690.25069808205776245No Hit
TACGGAAGGTCCTGGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA2820.191590404174225No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA2440.16577325751244998No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA2020.13723851646522497No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA2000.1358797192725No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA1920.1304445305016No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1840.12500934173069997No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1670.11345956559253749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGATAG109.16127E-4287.18497195
AGGCGAT158.037454E-6287.18497195
AAGACAG109.16127E-4287.18497195
AAGATAG27300.0287.18497195
AAGGCAT2900.0287.18497195
AAGGTAG109.16127E-4287.18497195
AAGCGGG2650.0287.18497195
CAGATAG109.16127E-4287.18497195
ACGATAG109.16127E-4287.18497195
AAGTTAG109.16127E-4287.18497195
AAGGCAG39250.0279.50232195
CAGGCAG202.533183E-5215.38872195
AGGCAGG900.0207.41136195
GGATGTC100.0029351537194.676864
GTCACAA750.0194.676868
ACGTCGG100.0029351537194.676862
AGGATGC100.0029351537194.676866
GGCGCGA16150.0194.676868
GGTCCTG204.8985567E-7194.676868
GCAGGTC100.0029351537194.676865
Produced by FastQC (version 0.11.5)