FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005302032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147189
Sequences flagged as poor quality0
Sequence length197-201
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2443016.59770770913587No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG2266415.397889787959699No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC1483610.079557575634048No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG140629.553703062049474No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG74155.037740592027936No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA66794.537703225105137No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA58553.9778787817024366No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC57853.930320879957062No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG41132.7943664268389625No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA25781.7514895814225249No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC23061.5666931632119248No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA22551.5320438347974374No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC21321.4484778074448499No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG20911.4206224649939874No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA12970.8811799794821624No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG10430.7086127360060873No Hit
CCTGTTTGCTCCCCTAGCTTTCGTACTTCAGCGTCAGTTACCGTCCAGTG9520.6467874637370999No Hit
CCCGTTCGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTTTCGTCCAGAA9420.6399934777734749No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA9250.6284437016353124No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA9180.623687911460775No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA8330.5659390307699624No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG7500.5095489472718749No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA7070.4803348076282875No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA6270.42598291991928744No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA6170.4191889339556625No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG6000.40763915781749993No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTCAGCGTCAGTTGCGCTCCCGTC5910.4015245704502375No Hit
CCTGTTCGCTCCCCACGCTTTCGTTCCTCAGCGTCAGTAACGGCCCAGAG5450.37027223501756246No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA5250.3566842630903125No Hit
CCTGTTCGATACCCGCACCTTCGCGCTTCAGCGTCAGTTGCGCTCCCGTC3610.2452628932868625No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG3500.23778950872687496No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA3500.23778950872687496No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGACCAGAG3190.21672815223963748No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTAATCGTCCAGAA2960.20110198452329997No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTTGCGCTCCCGTC2830.19226980277058747No Hit
CCCGTTCGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA2790.18955220838513748No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC2470.1678114533015375No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG2440.16577325751244998No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2160.14675009681429999No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1980.13452092207977498No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAATAGCCCAGAG1940.13180332769432498No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAA1910.1297651319052375No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTTTGGTCCAGCA1910.1297651319052375No Hit
CCTGTTTGATACCCGCACCTTCGTGCTTCAGCGTCAGTTGTGCTCCCGTA1860.126368138923425No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA1850.12568874032706248No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC1770.12025355155616249No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA1760.11957415295979999No Hit
CCGGTTCGCTCCCCACGCTTTCGTGCCTTAGCGTCAGAAATGGCCCAGTA1710.11617715997798748No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG1530.10394798524346249No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA1520.1032685866471No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGG350.0307.62515195
GTCACCG204.9934897E-8307.62515195
ATAACCG204.9934897E-8307.62515195
CCACCCG450.0307.62515195
GTAACCG204.9934897E-8307.62515195
ATATCCG800.0307.62515195
ATTGCCG156.1069695E-6307.62512195
AGACCGG156.1069695E-6307.62512195
CTGGCCG26200.0307.03806195
CCAGCCG3350.0303.03372195
CCAACCG13250.0301.8209195
TCGTCCG2800.0296.63852195
TCCACGG4700.0294.5347195
TAATCCG1150.0294.25012195
TCTCCGG10250.0279.11353195
CAAGCCG1550.0277.85495195
GAAGCCG4400.0265.67627195
CTTGCCG201.9251034E-5230.71886195
CCGGCCG402.5465852E-11230.71886195
TCCCCGG150.002510131205.08342195
Produced by FastQC (version 0.11.5)