FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005302048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88517
Sequences flagged as poor quality0
Sequence length198-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1903321.502084345379984No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC66517.5138109063795655No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG53726.068890721556311No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG53156.004496311442999No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG51385.804534722143769No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG46385.239671475535772No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG41614.7007919382717445No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA38644.365263169786595No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC30923.493114317023849No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG29383.3191364370685856No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC28943.2694284713670823No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA28223.1880881638555305No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG17071.928443123919699No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG10801.2201046126732717No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG9431.065332083102681No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA9321.052905091677305No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA8961.0122349379215292No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA7860.8879650236677701No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG7080.7998463571969227No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA6280.7094682377396433No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA6150.6947817933278353No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA5590.6315171097077398No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA4360.4925607510421727No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA4060.45866895624569287No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA3670.4146096230102692No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGGCCAGAG3130.3536043923766056No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA3060.3456963069240937No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA2710.30615587966153396No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTGTTGCCCAGAA2410.2722640848650542No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA2400.2711343583718382No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCCGTCCAGTG2290.2587073669464623No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA2270.2564479139600303No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTGTATCTTAGTG2210.24966955500073432No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA2090.2361128370821424No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA1810.20448049527209464No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA1720.1943129568331507No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1630.18414541839420678No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTAAGTTCTAGCA1400.15816170905023894No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGCCATCCCAGGG1360.15364280307737496No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA1220.13782663217235105No Hit
CCTGTTCGCTCCCCACGCTTTCGTCTCTCAGCGTCAATCGTCTGTTAGTC1160.13104827321305512No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTACCATCCCAGGG1040.1174915552944632No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTATTATCCCAGGG1010.11410237581481522No Hit
CCTGTTTGATACCCACGCCTTCGTGCTTCAGTGTCAGTCGCAGTATGGCA990.11184292282838326No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCCGTCCAGTG950.10732401685551929No Hit
ACCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA930.1050645638690873No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATAAATTGTTAGTA930.1050645638690873No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAATAGCCCAGAG900.10167538438943932No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGA890.10054565789622333No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC890.10054565789622333No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)