FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005302058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61960
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTCATCCCAGGA1946731.418657198192385No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG60559.772433828276307No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG59219.556165267914784No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG53628.653970303421563No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG24163.8992898644286638No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA18633.0067785668173017No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG17602.8405422853453843No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG16492.6613944480309875No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA16162.608134280180762No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC15132.441897998708844No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA13632.1998063266623626No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA11511.857650096836669No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA6170.9958037443511943No Hit
CCTGTTTGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG3530.5697224015493867No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTGAACGTCAGTGTCATCCCAGGA3450.5568108457069076No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTGTATCTTAGTG3170.5116204002582311No Hit
CCTGTTTGCTACCCACGCTTTCGCGCATGAACGTCAGTGTCATCCCAGGA2770.447062621045836No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACACGCCAGAG2130.3437701743060039No Hit
CCTGTTCGCTCCCCACGCTTTCGTCTCTCAGCGTCAATTGTCTGTTAGTC1870.30180761781794707No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG1860.3001936733376372No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACCTTCCCAAGG1810.2921239509360878No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTTTCATCCCAGGA1720.2775984506132989No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTATCATCCCAGGA1690.27275661717236926No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA1370.2211103938024532No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1360.2194964493221433No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATTAATTGTTAGTA1270.20497094899935442No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAGCGTCAGTGTCATCCCAGGA1240.20012911555842477No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA1190.1920593931568754No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG1180.19044544867656552No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTAACGTTACGGTA1120.18076178179470626No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTCATCCCAGTA950.15332472562943836No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCACGG870.14041316978695934No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTACATTCCCAAGG860.13879922530664945No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA790.1275016139444803No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG750.12104583602324079No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCATTGTCATCCCAGGA700.11297611362169142No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC680.10974822466107165No Hit
CCTGTTCGCTCCCCACGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG660.10652033570045191No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACCG105.6021527E-4338.1306195
TAGCCCG450.0338.1306195
CTGGCCG105.6021527E-4338.1306195
TATCCAG105.6021527E-4338.1306195
TAATCCG700.0338.1306195
AAGGCCG252.2919266E-10338.1306195
TTGGCCG105.6021527E-4338.1306195
CAGGCCG1950.0338.1306195
GTAACCG19500.0338.1306195
GGAACCG1250.0338.1306195
CAAACGG203.088462E-8338.1306195
AAGTCCG3200.0332.84732195
TAAACCA1450.0326.47095195
GTCACCG2650.0325.37097195
GAAACCG150.0018865564225.42043195
TCTCCAG3450.0220.51996195
GTTTGTT100.0029476995194.309134
TTGTTCC100.0029476995194.309136
CCTATTT100.0029476995194.309131
GTTTGCG153.813031E-5194.309134
Produced by FastQC (version 0.11.5)