FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005302090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147940
Sequences flagged as poor quality0
Sequence length194-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA2248615.199405164255781No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG133539.025956468838718No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA107327.254292280654319No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG101196.839935108827904No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG86185.825334595106124No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG79635.38258753548736No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA75205.08314181424902No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC73874.993240502906584No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG71044.801946735162904No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG70444.7613897526024065No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG46573.1478977964039476No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG32922.2252264431526294No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA30632.070433959713397No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC29481.9926997431391105No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC29251.9771528998242531No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA19141.2937677436798702No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA17411.176828443963769No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA15611.0551574962822765No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA11050.7469244288224955No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA10500.7097471948087062No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG9660.6529674192240097No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG6420.43395971339732325No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA6410.4332837636879816No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG6130.4143571718264161No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA5550.37515208868460187No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA4980.3366229552521292No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG4980.3366229552521292No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA4790.32377991077463836No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG4690.31702041368122214No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA3640.2460456942003515No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA3510.23725834797891035No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA3310.22373935379207785No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC3190.21562795727997838No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3060.20684061105853724No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACCTTCCCAAGG2780.18791401919697176No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGCTATCCCAGGG2530.17101527646343112No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC2330.15749628227659862No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA2300.15546843314857375No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTGTATCTTAGTG2250.15208868460186561No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTGTTGCCCAGAA2000.135189941868325No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTTGGCTAGCA1950.13181019332161686No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG1940.13113424361227527No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA1560.1054481546572935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCCG500.0356.98575195
GCAACCG104.772833E-4356.98575195
TCTGCGG153.3694614E-6356.98575195
ATAGCCG202.3743269E-8356.98575195
TCGTCCG350.0356.98575195
AAAACCG104.772833E-4356.98575195
GGAACCG3350.0356.98575195
CAAACGG202.3743269E-8356.98575195
ATATCCG1150.0356.98575195
CTGGCCG11350.0356.98572195
GTAACCG13200.0355.6335195
GTCACCG12400.0352.66733195
CCAGCCG6050.0351.0851195
TAAACCA2100.0348.48608195
CCAACCG4100.0348.27878195
TAATCCG1350.0330.54236195
TCTCCGG4550.0309.91068195
CAAGCCG1450.0307.7463195
GAAGCCG1100.0275.8526195
AGGGCCG150.0016074581237.9905195
Produced by FastQC (version 0.11.5)