FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005302094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114070
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1767415.493994915402823No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG1222610.717980187604102No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG65475.739458227404225No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC62485.477338476374157No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC55534.868063469799246No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG43383.8029280266503025No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC42173.696852809678268No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA36433.1936530200753923No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG32782.8736740597878496No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTATCGTCCAGTA32732.869290786359253No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA29142.5545717541860262No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCTGTCCAGTG28482.4967125449285525No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG26362.310861751556062No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA25392.22582624704129No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA24782.1723503112124134No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG22541.9759796616112915No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA21291.8663978258963794No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA19221.6849303059524854No Hit
CCTGTTTGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG15031.3176119926361007No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA11841.0379591478916455No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA10000.8766546857192952No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG9260.8117822389760674No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC8590.7530463750328745No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA8210.7197334969755413No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGCGTCAGTTGTTGCCCAGAA7850.6881739282896467No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA7780.6820373454896117No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA7020.6154115893749452No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG5660.49618655211712104No Hit
CCTGTTTGCTCCCCTCGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG5010.4392039975453669No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA4930.43219076005961254No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA4460.3909879898308056No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG4350.38134478828789337No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA4020.35241518365915664No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG3780.3313754712018936No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGCA3610.3164723415446656No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTC3540.3103357587446305No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTATCGTCCAGTG3400.29806259314456035No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGAA3200.28052949943017447No Hit
CCTATTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTTGGCTAGCA3120.2735162619444201No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA3040.26650302445866575No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA2550.22354694485842028No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA2000.17533093714385903No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA1940.17007100902954325No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA1890.1656877356009468No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1840.1613044621723503No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACACGCCAGAG1700.14903129657228018No Hit
CCGGTTCGCTCCCCACGCTTTCGTGCCTTAGCGTCAGAAATGGCCCAGTA1580.13851144034364862No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTATATCTTAGTA1510.13237485754361356No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC1470.1288682388007364No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATCGTCCAGTT1400.12273165600070132No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGCCATCCCAGGG1370.12010169194354343No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC1340.11747172788638555No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA1210.10607521697203473No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGA1160.10169194354343823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGCCG1500.0315.9566195
TAGCCCG155.48326E-6315.9566195
TTGACCG155.48326E-6315.9566195
AAAACCG155.48326E-6315.9566195
GTAACCG2950.0315.9566195
GGAACCG700.0315.9566195
GAAACCG106.878465E-4315.95657195
GTACCCG106.878465E-4315.95657195
AGGGCCG106.878465E-4315.95657195
TATCCGG106.878465E-4315.95657195
ATTGCCG106.878465E-4315.95657195
TCGTCCG800.0315.95657195
CTGGCCG15600.0314.9439195
CCAACCG3250.0311.09573195
GTCACCG2900.0310.50903195
ATATCCG2850.0304.87036195
GAAGCCG2300.0288.48212195
TAATCCG1050.0285.86548195
TCTGCGG400.0276.462195
CAAGCCG3800.0274.38333195
Produced by FastQC (version 0.11.5)