FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005302096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139973
Sequences flagged as poor quality0
Sequence length198-201
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA1570611.22073542754674No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1404610.034792424253249No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG126219.016738942510342No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC125428.960299486329507No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGTACCTTAGTA103577.399284147657048No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA97466.962771391625528No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG83105.936859251427061No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA52733.7671550941967378No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG46953.3542183135318955No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG39542.8248305030255834No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA29782.127553170968687No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG21221.5160066584269822No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCAGCCCGGAC17531.2523843884177663No Hit
CCTGTTCGATACCCGCACCTTCGTGCTTAAGCGTCAGTAACGTTACGGTA15801.1287891236166974No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATTAATTGTTAGTA14881.0630621619883835No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA14061.0044794353196689No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG13190.9423245911711545No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA11000.7858658455559285No Hit
CCTGTTCGCTCCCCACGCTTTCGTCTCTCAGCGTCAATTGTCTGTTAGTC10650.760861023197331No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA10580.7558600587256114No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA10040.7172811899437749No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG8890.6351224879083823No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA6670.4765204718052768No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG6450.4608031548941582No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTATTATCCCAGGG6030.4307973680638409No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTAAGTTCCAGCA4330.3093453737506519No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTGAGCGTCAGTAAAAGTCCAGAA3990.2850549748880141No Hit
CCTGTTCGCTCCCCACGCTCTCGTCTCTCAGCGTCAATCATTTGTTAGTC3940.28148285740821444No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC3160.2257578247233395No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACACGCCAGAG3000.21432704878798053No Hit
CCTGTTCGATACCCACGCTTTCGAGCATCAGCGTCAGTTGCGCTACAGTA2810.20075300236474178No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATAAATTGTTAGTA2780.19860973187686196No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTGTATCTTAGTG2670.19075107342130268No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACCTTCCCAAGG2630.18789337943746293No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA2590.1850356854536232No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA2520.18003472098190365No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTAACGCTCCCGCA2510.17932029748594372No Hit
CCTGTTCGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTGCCGTCCAGTG2220.1586020161031056No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA2210.15788759260714566No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTTCCGTCCAGTG1930.13788373472026746No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA1910.1364548877283476No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTGAACGTCAGTGTTATCCCAGGA1910.1364548877283476No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGG1750.12502411179298867No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA1660.11859430032934923No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA1560.11145006536974987No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGTTATCCCAGGA1550.11073564187378995No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGAA1540.11002121837783001No Hit
CCTGTTTGCTACCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG1520.10859237138591013No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG1440.10287698341823066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTCCG154.7532394E-6327.52194195
GAAACCG252.8194336E-10327.52194195
CCAGCCG203.650166E-8327.52194195
CTGGCCG301.8189894E-12327.52194195
TCTGCGG106.178292E-4327.52194195
ATAGCCG203.650166E-8327.52194195
TATCCCG106.178292E-4327.52194195
ATTGCCG1000.0327.52194195
TCTCCCG106.178292E-4327.52194195
GTAACCG18500.0327.52194195
CAAACGG1600.0327.52194195
ACAACCG106.178292E-4327.52194195
CATGCCG252.8194336E-10327.52194195
GTCACCG11550.0326.1041195
GGAACCG1850.0318.67195
TAAACCA6300.0317.12442195
TAGCCCG4150.0303.84567195
CAGGCCG12300.0292.90582195
ATAACCG357.2759576E-12280.7331195
GCAACCG1650.0277.8974195
Produced by FastQC (version 0.11.5)