FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005302097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91729
Sequences flagged as poor quality0
Sequence length198-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA1486316.203163666888333No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA1388615.13806974893436No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1193013.005701577472772No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA74718.144643460628592No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA72757.930970576371704No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA62326.793925585147555No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA51015.56094582956317No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA38674.215678792966237No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA19182.0909417959423955No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA18171.9808348504834892No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA17871.948129817178864No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA15491.6886698862955007No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA14791.612358141918041No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATCATTGGGCGTAAAGGGAGCGCA11491.2526027755671598No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA9911.080356266829465No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA6890.7511255982295675No Hit
TACGCAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA4910.5352723784190387No Hit
TACGGAAGGTCCTGGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA3920.4273457685137743No Hit
TACGCAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA3770.4109932518614615No Hit
TACGTAGGTGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA2650.28889446085752596No Hit
TACGTAGGGGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA2260.24637791756151273No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1890.20604170981914116No Hit
TACGCAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA1770.19295969649729094No Hit
TACGCATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA1730.1885990253900075No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGTGCGTA1690.18423835428272411No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA1450.15807432763902365No Hit
TACGCAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1410.15371365653174024No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA1330.1449923143171734No Hit
TACGTAGGGCGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGCTTGTA1180.12863979766486064No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1090.11882828767347295No Hit
TACGTAGGTCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA1060.11555778434301038No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA1050.11446761656618953No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA1040.11337744878936867No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA1020.11119711323572697No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA1010.11010694545890612No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGTGCGTA980.10683644212844358No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGCGCGCA920.10029543546751846No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGGTTAAAGGGAGCGTA920.10029543546751846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGATAG8450.0255.86821195
AAGGCAT700.0255.86821195
AGGCAGG301.2732926E-11255.8682195
AAGCTAG201.2494093E-7255.8682195
AAGGCAG33950.0253.23038195
AAGGCGG24350.0235.90312195
GAGGGCA253.80056E-7204.69455195
GTATGTC8300.0194.761184
GAGCGAG153.7837613E-5194.761189
GGGCAAG4800.0194.761189
ACGCATG308.185452E-11194.761182
GTCGCGA2350.0194.761188
GTCGCAA6650.0194.761188
GCAGGTG256.320988E-9194.761184
GCAGGTC308.185452E-11194.761184
GGTCCCG6250.0194.761187
TACGGAG450.0194.761181
CGTAGGG28350.0194.761183
GTCCCGA6250.0194.761188
TACGTAT8350.0194.761181
Produced by FastQC (version 0.11.5)