FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005302114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201526
Sequences flagged as poor quality0
Sequence length197-201
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3781718.76532060379306No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG3469017.21365977590981No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2465012.231672340045453No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG161388.007899725097506No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG128706.38627273900142No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGTA78663.9032184432777903No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG68993.4233796135486236No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG63153.1335907029365937No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC57712.8636503478459354No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG37951.883131705090162No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC28791.428599783650745No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA21581.0708295703780157No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA19450.9651360122267102No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG18840.9348669650566179No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG18450.9155146234232805No Hit
CCCGTTCGCTTCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA17530.8698629457241249No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTGTCGTCCAGTA11870.589005885096712No Hit
CCTGTTTGCTCCCCACGCCTTCGAGCCTCAACGTCAGTTTTCGTCCAGCA11660.5785853934479919No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTGATCGTCCAGAA10190.5056419519069499No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTATTGTCCAGTA9430.46792969641634324No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC8890.44113414646249116No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG8450.41930073538898205No Hit
CCTGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA7800.3870468326667527No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGTGCTCCCGTA6840.33941029941546No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG6660.33047844943084265No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTGTCCCAGGA6600.32750116610263685No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG5090.25257286900945786No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTTACACTCCCGTA4680.23222809960005159No Hit
CCCGTTCGCTCCCCTCGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3810.18905749134106767No Hit
CCTGTTCGCTCCCCACGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG3790.18806506356499905No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG3580.17764457191627878No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA3480.1726824330359358No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTGAGTTCCAGCA3480.1726824330359358No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA3320.16474301082738704No Hit
CCCGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG3200.15878844417097546No Hit
CCTGTTTGCTCCCCTAGCTTTCGCTCCTCAGCGTCAGTTATGGCCCAGAA3040.1508490219624267No Hit
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGACCAGAG2800.13893988864960352No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG2560.12703075533678038No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGACCAGAG2490.12355725812054028No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTTTGGTCCAGCA2190.10867084147951132No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG2170.10767841370344274No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGCCCAGAG2170.10767841370344274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACCG1100.01091.3976195
ATTTCCG153.8562575E-81091.3976195
AATCCGA50.00686362251091.3975195
CCAGCCA50.00686362251091.3975195
GACACCG50.00686362251091.3975195
CTGGCCG650.01091.3975195
ATTGCCG450.01091.3975195
GATCCGG101.6710761E-51091.3975195
TAATCCG350.01091.3975195
AATGCCG50.00686362251091.3975195
TGTACCG50.00686362251091.3975195
CAAACGG50.00686362251091.3975195
CCAGCCG3600.01045.9226195
GGAACCG1200.01045.9226195
GTCACCG3800.01033.9554195
CCAACCG4350.01028.6735195
TAAACCA2050.01011.53906195
GAAGCCG400.0954.97284195
CATTCCG2100.0935.4836195
TTCGCCG1850.0855.41974195
Produced by FastQC (version 0.11.5)