FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005302122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81670
Sequences flagged as poor quality0
Sequence length197-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCATGCTTTCGCTTCTCAGCGTCAGTTACAGCCCAGAG1805822.11093424758173No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG1759821.547691930941596No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG80339.83592506428309No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG61917.580506918084976No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG46655.71201175462226No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGGCCAAAA45585.580996694012489No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA34514.225541814619811No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGCTATCCCAGGG16902.0693032937431126No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG10391.27219297171544No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTGTTATCCCAGGA8741.0701604016162607No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTGTTATCCCAGGA7800.9550630586506674No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG6960.852210113872903No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACAGCCCAGAG5350.6550753030488551No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTACACGGCCCAGA5230.6403820252234602No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACACGCCAGAG5210.6379331455858944No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTGACAGACCAGAG4820.5901799926533611No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTC4610.5644667564589201No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA4490.5497734786335252No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATTAATTGTTAGTA3040.3722297049100037No Hit
CCTGTTTGATACCCGCACCTTCGAGCTTAAGCGTCAGTTGCGCTCCCGTC2610.3195787927023387No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGTGCTCCCGCA2550.3122321537896412No Hit
CCTGTTTGCTCCCCACGCTTTCACGCATTAGCGTCAGTTAAGTTCCAGCA2260.2767233990449369No Hit
CCTGTTCGCTCCCCACGCTTTCGTCTCTCAGCGTCAATTGTCTGTTAGTC2210.2706011999510224No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTAAGCGTCAGTAACACTCCCGCA2030.24856128321293006No Hit
CCTGTTTGCTCCCCTAGCTTTCGCACTTCAGCGTCAGTTACCGTCCAGTG1990.24366352393779844No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG1660.20325700991796256No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACACCCCGGAC1570.19223705154891638No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAAGCCAGAG1540.18856373209256766No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATAAATTGTTAGTA1530.18733929227378474No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACCTTCCCAAGG1480.1812170931798702No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTGTATCTTAGTG1470.1799926533610873No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGACAGACCAGAG1340.16407493571690954No Hit
CCTGTTCGCTCCCCCGGCTTTCGCACTTCAGCGTCAGTTTCCGTCCAGTG1280.15672829680421207No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGA1250.15305497734786336No Hit
CCCGTTCGCTCCCCTGGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA1190.1457083384351659No Hit
CCTGTTTGCTACCCACGCTTTCGAGCATGAACGTCAGTATTATCCCAGGG1050.12856618097220524No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAATCCAGAG960.11754622260315906No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG940.11509734296559324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACCG4450.0598.7378195
AGGGCCG101.00949655E-4598.73773195
CTGGCCG101.00949655E-4598.73773195
CAGACCG300.0598.73773195
GGAACCG300.0598.73773195
CGACCCG101.00949655E-4598.73773195
TAGGCCG201.7735147E-9598.73773195
GAAACCG257.2759576E-12598.7377195
ATAGCCG257.2759576E-12598.7377195
TAATCCG257.2759576E-12598.7377195
CCAACCG4500.0592.08514195
TAAACCA3950.0591.1588195
GTCACCG6100.0588.92236195
AAGTCCG950.0535.7127195
CCAGCCG650.0460.5675195
ATAACCG201.3374301E-6449.0533195
CAGCCCG450.0399.15848195
TCAACCA153.4028108E-4399.15848195
CGAACCG153.4028108E-4399.15848195
AAAACCG301.342778E-8399.15848195
Produced by FastQC (version 0.11.5)