FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005302125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383
Sequences flagged as poor quality0
Sequence length199-201
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACGAAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA13234.46475195822455No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA6918.01566579634465No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA184.699738903394255No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA164.177545691906006No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA123.1331592689295036No Hit
TACGTAGATGGCGAGCGTTACCCGGATTTATTGGGCGTAAAGAGGGCGCA102.610966057441253No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA92.3498694516971277No Hit
TACGGAGGGAGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTA82.088772845953003No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA71.8276762402088773No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA71.8276762402088773No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA61.5665796344647518No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA61.5665796344647518No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA51.3054830287206265No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA51.3054830287206265No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA51.3054830287206265No Hit
TACGTAGGGCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGCTCGTA41.0443864229765014No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA41.0443864229765014No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA30.7832898172323759No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA30.7832898172323759No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA30.7832898172323759No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA30.7832898172323759No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA20.5221932114882507No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA20.5221932114882507No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA20.5221932114882507No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA20.5221932114882507No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA20.5221932114882507No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA20.5221932114882507No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA20.5221932114882507No Hit
TACGAAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA20.5221932114882507No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGGGTGCGTA20.5221932114882507No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA10.26109660574412535No Hit
TACGGATGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA10.26109660574412535No Hit
TACGGAAGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA10.26109660574412535No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA10.26109660574412535No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA10.26109660574412535No Hit
GACGAAGGATGCAAGCGTTATCCATTCATTGGGTTTAAAGAGAGCGTGGG10.26109660574412535No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA10.26109660574412535No Hit
TACGGAGGGAGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCATGTA10.26109660574412535No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA10.26109660574412535No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA10.26109660574412535No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCTCGCA10.26109660574412535No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATCACTGGGCGTAAAGGACGCGTA10.26109660574412535No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGCGTA10.26109660574412535No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGCGTAAAGGGCTCGTA10.26109660574412535No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGAGCGTA10.26109660574412535No Hit
TACGTAGGTTCCGAGCGTTATCCGGATTTATTGGGCTTAAAGCGAGCGCA10.26109660574412535No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA10.26109660574412535No Hit
TACGTCGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAATCGCGCGCA10.26109660574412535No Hit
TACGTATGTCGCAAGCGTTATCCGGATTTATTGGGCATAAAGGGAACCTA10.26109660574412535No Hit
TACGTAGGTTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA10.26109660574412535No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTATTGGGTGTAAAGGGTGCGTA10.26109660574412535No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGACGTAAAGCGCGCGTA10.26109660574412535No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGCGCGTA10.26109660574412535No Hit
TACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGGGTGCGTA10.26109660574412535No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA10.26109660574412535No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA10.26109660574412535No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA10.26109660574412535No Hit
TACGTATGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA10.26109660574412535No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA10.26109660574412535No Hit
TACGGAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA10.26109660574412535No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATGCA207.3985575E-8195.07
GTGCAAG100.0017958507195.09
AGGATGC207.3985575E-8195.06
GATGCAA207.3985575E-8195.08
ACGTAGG100.0017958507195.02
CGAAGGA207.3985575E-8195.03
CGTAGGG100.0017958507195.03
TACGTAG100.0017958507195.01
GACGAAG207.3985575E-8195.01
AAGGATG207.3985575E-8195.05
GTAGGGT100.0017958507195.04
TAGGGTG100.0017958507195.05
ACGAAGG207.3985575E-8195.02
GGGTGCA100.0017958507195.07
GGTGCAA100.0017958507195.08
GAAGGAT207.3985575E-8195.04
AAGGCAG300.0194.99998195
ATGCAAG252.2551285E-7156.09
AGGGTGC150.0060476754129.999986
TGAGAAA200.001068132639.090-94
Produced by FastQC (version 0.11.5)