FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005302127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences575
Sequences flagged as poor quality0
Sequence length200-201
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA29150.60869565217392No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA295.043478260869565No Hit
TACGTAGGAGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGAGCGCGTA274.695652173913043No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA223.826086956521739No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA183.130434782608696No Hit
GACGAAGGATGCAAGCGTTATCCGGATTCATTGGGTTTAAAGGGAGCGTA152.608695652173913No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA132.2608695652173916No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA91.565217391304348No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA81.391304347826087No Hit
TACGGAAGGTCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA81.391304347826087No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA71.2173913043478262No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA71.2173913043478262No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGCGCGTA71.2173913043478262No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA71.2173913043478262No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA71.2173913043478262No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA71.2173913043478262No Hit
TACGTAGGGCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA61.0434782608695654No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA50.8695652173913043No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA50.8695652173913043No Hit
TACGGAAGGTTCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA50.8695652173913043No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAAAGGGCATCTA50.8695652173913043No Hit
TACGGAAGGTCCGGGCGTTATCCGGAATTATTGGGTTTAAAGGGAGCGCA40.6956521739130435No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA30.5217391304347827No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA20.34782608695652173No Hit
TACGTAGGTTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA20.34782608695652173No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA20.34782608695652173No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGCGTCTA20.34782608695652173No Hit
TACGGAGGATGCGAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA20.34782608695652173No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTTGTA20.34782608695652173No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGCGTAAAGCGAGCGTA10.17391304347826086No Hit
TACGTAGGTTGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA10.17391304347826086No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAGAGCGTGCGCA10.17391304347826086No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA10.17391304347826086No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCATAAAGCGCATCTA10.17391304347826086No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGTGCGTCTA10.17391304347826086No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA10.17391304347826086No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA10.17391304347826086No Hit
TACGGAAGGTCCAGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGTGTA10.17391304347826086No Hit
TACGTAGGTCCCGAGCGTTGTCTGGATTTATTGGGCGTAAAGCGAGCGCA10.17391304347826086No Hit
TACGTAGGTGGCGAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA10.17391304347826086No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA10.17391304347826086No Hit
TACGTAGGTGGCGAGCGTTGTCCGGATTTACTGGGCGTAAAGGGAGCGTA10.17391304347826086No Hit
TACGTAGAGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA10.17391304347826086No Hit
TACGTATGTCGCGAGCGTTATCCGGAATTATTGGGCATAATGGGCATCTA10.17391304347826086No Hit
TACGTAGGGCGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGCTCGCA10.17391304347826086No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA10.17391304347826086No Hit
TACGTATGTCACAAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGTGTA10.17391304347826086No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA10.17391304347826086No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCTTAAAGGGCTTCTA10.17391304347826086No Hit
TACGTAGGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGAGTA10.17391304347826086No Hit
TACGTAGGTCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTCGTA10.17391304347826086No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA10.17391304347826086No Hit
TACGGAAGGTCCTGGTGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA10.17391304347826086No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA10.17391304347826086No Hit
TACGTAGGGTGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGCTCGTA10.17391304347826086No Hit
TACGGAGGATCCGGGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA10.17391304347826086No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGGGAGCGTA10.17391304347826086No Hit
TCCGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGACCGCA10.17391304347826086No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA10.17391304347826086No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTCAAGCGTGCGCA10.17391304347826086No Hit
TACGTAGGGCGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGCGCGTA10.17391304347826086No Hit
TACGTAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGCA10.17391304347826086No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGGGCGCA10.17391304347826086No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCGCGCA10.17391304347826086No Hit
TACGGAGGGTGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGCGCGTA10.17391304347826086No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA10.17391304347826086No Hit
TACGTATGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA10.17391304347826086No Hit
TACGTAGGTCTCAAGCGTTATCCGGATTTACTGGGCGTAAAGTGTCCGTA10.17391304347826086No Hit
TACGTAGGTCGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA10.17391304347826086No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGCA10.17391304347826086No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCTTGTA10.17391304347826086No Hit
TACAGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGTGTA10.17391304347826086No Hit
TACGTAGGAGGCGAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCGCGTA10.17391304347826086No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA10.17391304347826086No Hit
TACGTATGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCACGTA10.17391304347826086No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCATAAAGGGCATCTA10.17391304347826086No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGTGCA10.17391304347826086No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)