FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005302278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80330547
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAGTGATCTCGTAT955908111.899683690688674TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAGTGATGTCGTAT46952015.84485127432283TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAGTGATGGCGTAT3732720.4646700588258163TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAGTGATCGCGTAT2997170.37310464224773676TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAGTGATGTCGTTT925540.11521644437451671TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAGTGATCTCGTTT918360.11432263743952845TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAGTGATCTCCTAT894890.1114009593386685TruSeq Adapter, Index 19 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAGTGATCTCGTAA859510.10699665719940885TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)