FastQCFastQC Report
Thu 12 May 2022
EGAF00005302298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46720496
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC40421988.651873045183423TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC21348454.569397122838764TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGGCGTATGC2052870.43939387972250976TruSeq Adapter, Index 5 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATTC1299870.2782226455815024TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCGCGTATGC1228710.2629916428969419TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATTC1181000.2527798506248735TruSeq Adapter, Index 5 (97% over 48bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGGATGC540580.11570510724029984TruSeq Adapter, Index 5 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTTTGC492680.10545264759175502TruSeq Adapter, Index 5 (97% over 46bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGGATGC479530.1026380370619353TruSeq Adapter, Index 5 (97% over 45bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)