FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005302308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84750694
Sequences flagged as poor quality0
Sequence length150
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGCTAGATCTCGTAT3490866841.18983143666057TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGCTAGATCGCGTAT2418680.28538763352191543TruSeq Adapter, Index 4 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGAGCTAGATCTCGTATGCC2126820.2509501574111004TruSeq Adapter, Index 4 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTGAGCTAGATCGCGTATGCC887030.1046634497175917TruSeq Adapter, Index 4 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGAGCTCGATCTCGTAT884540.10436964681374762TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACTGAGCTAGATCTCGTATG850420.10034372107914538Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)