FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005302318

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302318
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177291223
Sequences flagged as poor quality0
Sequence length150
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGAATCTCGTAT5121544428.887749282433457TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGAATCTCGGAT145880268.228284374799536TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGAATCTCGTTT31751081.7908997108108393TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGAATCTCGTGT20344531.147520427449474TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGAATCTCGGTT10860410.6125745999281645TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGAATCTCGGGT7939320.4478123544784843TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAAGAATCGCGTAT2061470.11627591964888188TruSeq Adapter, Index 20 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)