FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005302320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81457335
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCGTATCTCGTAT23421372.8752929370940015TruSeq Adapter, Index 7 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCGTATCGCGTAT7379580.9059441976588112TruSeq Adapter, Index 7 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCGTATCTGGTAT3717830.4564143916566875TruSeq Adapter, Index 7 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCGTATCGGGTAT2515720.3088389768705298TruSeq Adapter, Index 7 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCGTATCGCGGAT1844130.2263921352202352TruSeq Adapter, Index 7 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCGTATCTCGGAT1670260.2050472188907236TruSeq Adapter, Index 7 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCGTATCTCGTTT1367330.16785842551809482TruSeq Adapter, Index 7 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGATCGTATCGGGGAT1098260.13482640943261892TruSeq Adapter, Index 7 (97% over 39bp)

[OK]Adapter Content

Adapter graph