FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005302324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139491228
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTAATATCTCGTAT1907272613.67306480375956TruSeq Adapter, Index 7 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTAATATCTCGTATGCC8890310.6373382848131497TruSeq Adapter, Index 7 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTAATATCGCGTATGCC3867020.2772231670367114TruSeq Adapter, Index 7 (96% over 33bp)
GATCGGAAGAGACACGTCTGAACTCCAGTCACCGGCTAATATCTCGTATG2061920.1478171803032661TruSeq Adapter, Index 2 (95% over 24bp)
GATCGGAAGAGCACAGTCTGAACTCCAGTCACCGGCTAATATCTCGTATG2003580.1436348384573688No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)