FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005302342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005302342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23319886
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCAACGCATCTCGTATG461610.1979469367903428TruSeq Adapter, Index 6 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCAACGCATCTCGTAT246740.10580669219394984TruSeq Adapter, Index 6 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA130150.053.1894571
TCGGAAG146500.046.8813672
CGGAAGA154800.044.306343
GATCGGA54350.044.066291
TCGTATG109350.039.28795244-45
TCTCGTA108450.038.6066742-43
AACGCAT110750.037.78346336-37
ACACGTC133900.034.62093712-13
CCAACGC129000.034.260734-35
CGCATCT123450.033.89646538-39
ATGCCGT102950.033.7949548-49
ACGTCTG152250.030.43263414-15
GTATGCC142800.030.3510946-47
CATCTCG139800.030.25494440-41
TATGCCG104550.029.34797146-47
GCCGTCT113700.029.28390750-51
CTCGTAT105850.029.2343342-43
CGTATGC107600.028.89129344-45
CGTCTTC120400.027.20076452-53
AGAGCAC258900.027.1885327
Produced by FastQC (version 0.11.5)