FastQCFastQC Report
Thu 12 May 2022
EGAF00005304594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79987882
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9071061.1340542808721952TruSeq Adapter, Index 16 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1039070.12990342712162326TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)