FastQCFastQC Report
Thu 12 May 2022
EGAF00005304614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005304614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49858836
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA11552242.3169895101442TruSeq Adapter, Index 10 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA10617322.12947610730423TruSeq Adapter, Index 10 (100% over 63bp)
CTTGACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACCTCTGGGTCCA901010.1807122011432437No Hit
GTGCTTTATGGCATCTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTGCCCATGATGGC722210.14485095480367813No Hit
CCTTGACTTTGGGGTTGCCCATGATGGCAGAGGCAGAGGACAGGTTGCCAAAGCTGTCAAAGAACCTCTGGGTCC630270.12641089334696862No Hit
GGGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGC629900.12633668383273128No Hit
GTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTATGGCATCTCCCAAGGAAGTCAG626970.12574902470647328No Hit
GTCACAGTGCAGTTCACTCAGCTGGGCAAAGGTGCCCTTGAGATCATCCAGGTGCTTTATGGCATCTCCCAAGGA584210.11717281165569127No Hit
GGCATGTCGTCCACGTGCGCCACGGCGTTGGTCAGCGCGTCGGCCACCTTCTTGCCGTGGCCCTTAACCTGGGCA557280.11177156241673994No Hit
GGTGCTTTATGGCATCTCCCAAGGAAGTCAGCACCTTCTTGCCATGTGCCTTGACTTTGGGGTTGCCCATGATGG526440.10558609912192897No Hit
GGCTGAGATGCTTTTAAATGTGATGTTATAAGCCTAAGGCAGCTTGACTTGCAGCAACAAGTTTTTTACCCTCCT521210.10453713760987118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)